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Neural Mechanisms

Gene Transfer of Neuronal Nitric Oxide Synthase into Intracardiac Ganglia Reverses Vagal Impairment in Hypertensive Rats

Daniel A. Heaton, Dan Li, Susanna C. Almond, Tom A. Dawson, Lijun Wang, Keith M. Channon, David J. Paterson
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https://doi.org/10.1161/01.HYP.0000255792.97033.f7
Hypertension. 2007;49:380-388
Originally published January 18, 2007
Daniel A. Heaton
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Dan Li
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Susanna C. Almond
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Tom A. Dawson
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Lijun Wang
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Keith M. Channon
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David J. Paterson
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Abstract

Hypertension is associated with reduced cardiac vagal activity and decreased atrial guanylate cyclase and cGMP levels. Neuronal production of NO facilitates cardiac parasympathetic transmission, although oxidative stress caused by hypertension may disrupt this pathway. We tested the hypothesis that peripheral vagal responsiveness is attenuated in the spontaneously hypertensive rat (SHR) because of impaired NO-cGMP signaling and that gene transfer of neuronal NO synthase (nNOS) into cholinergic intracardiac ganglia can restore neural function. Cardiac vagal heart rate responses in the isolated SHR atrial/right vagus preparation were significantly attenuated compared with age-matched normotensive Wistar–Kyoto rats. [3H] acetylcholine release was also significantly lower in the SHR. The NO donor, sodium nitroprusside, augmented vagal responses to nerve stimulation and [3H] acetylcholine release in the Wistar–Kyoto rat, whereas the soluble guanylate cyclase inhibitor 1H-(1,2,4)oxadiazolo(4,3-a)quinoxaline-1-one attenuated [3H] acetylcholine release in Wistar–Kyoto atria. No effects of sodium nitroprusside or 1H-(1,2,4)oxadiazolo(4,3-a)quinoxaline-1-one were seen in the SHR during nerve stimulation. In contrast, SHR atria were hyperresponsive to carbachol-induced bradycardia, with elevated production of atrial cGMP. After gene transfer of adenoviral nNOS into the right atrium, vagal responsiveness in vivo was significantly increased in the SHR compared with transfection with adenoviral enhanced green fluorescent protein. Atrial nNOS activity was increased after gene transfer of adenoviral nNOS, as was expression of α1-soluble guanylate cyclase in both groups compared with adenoviral enhanced green fluorescent protein. In conclusion, a significant component of cardiac vagal dysfunction in hypertension is attributed to an impairment of the postganglionic presynaptic NO-cGMP pathway and that overexpression of nNOS can reverse this neural phenotype.

  • NO
  • gene transfer
  • autonomic nervous system
  • acetylcholine
  • heart rate
  • hypertension
  • guanylate cyclase

Cardiac autonomic imbalance has been implicated in the etiology of hypertension and is characterized by sympathetic overactivity that is coupled with depressed baroreflex-mediated cardiac vagal responsiveness.1,2 Reduced cardiac vagal tone has been demonstrated in hypertensive patients3,4 and in hypertensive animal models.5,6 Moreover, similar autonomic responses are observed in normotensive subjects with a family history of hypertension,7 suggesting that parasympathetic dysfunction may be an early feature of the pathophysiology of this disease. This is clinically significant, because impaired cardiac vagal function is a powerful independent predictor of mortality.8,9 In addition, vagal activation exerts strong antiarrhythmic effects during coronary artery occlusion in exercising dogs10 and may improve survival in rats after myocardial infarction.11

The mechanisms responsible for cardiac vagal impairment in hypertension remain elusive. However, it is now widely established that reduced bioavailability of NO, associated with increased oxidative stress, is characteristic of the hypertensive phenotype.12,13 Moreover, downregulation of soluble guanylate cyclase (sGC), the key mediator of cGMP-dependent effects of NO, is observed in the aorta and atria of the spontaneously hypertensive rat (SHR).14,15 Under normal conditions, NO derived from neuronal NO synthase (nNOS) facilitates vagal neurotransmission and bradycardia, via a cGMP-dependent pathway.16 Emerging evidence shows that adenoviral gene transfer of nNOS to cardiac vagal neurons within the cervical vagus and right atrium in normotensive animals results in a rapid enhancement of parasympathetic function.17,18 nNOS gene transfer also normalizes hyperresponsiveness to β-adrenergic stimulation in the sino-atrial node of the SHR.15 We tested the hypothesis that impaired NO-cGMP signaling in hypertension attenuates peripheral vagal responsiveness in the SHR. From this we further hypothesized that overexpression of nNOS in postganglionic cholinergic neurons would restore peripheral parasympathetic function.

Methods

Animal Care

The investigation conformed to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (National Institutes of Health Publication No. 85-23, revised 1996) and the Animals (Scientific Procedures) Act 1986 (United Kingdom) and was performed under British Home Office license requirements (PPL 30/2130). Age-matched (16 to 24 weeks old) male SHR and Wistar–Kyoto (WKY) rats were housed under standard laboratory conditions.

Isolated Rat Sino-Atrial Node/Right Vagus Nerve Preparation: Assessment of Vagal Function

Dissection

Animals were killed by exsanguination under deep halothane anesthesia (4% in 100% O2). The heart was exposed and the ventricles removed, allowing the atria to be back perfused with 10 mL of heparinized (1000 U/mL) Tyrode’s solution. The thorax and mediastinum were rapidly removed and placed in oxygenated (95% O2 and 5% CO2) Tyrode’s solution at room temperature in a Perspex dissecting dish with a Sylgard base. The atria and right vagus were carefully separated from surrounding tissues, and the proximal end of the right vagus was tied off.

Experimental Preparation

Sutures (Ethicon, 5/0 Mersilk) were placed at the lateral edges of both atria, and the preparation was transferred to a preheated (37±0.1°C) water-jacketed organ bath containing 100 mL of continuously oxygenated Tyrode’s solution. The atria were vertically mounted with the suture in the left atrium connected to a stainless steel hook and the suture in the right atrium attached to an isometric force transducer (Harvard Apparatus, model 60-2997) connected to an amplifier. Heart rate was triggered from contraction and recorded in real time (Biopac MP100 with Acqknowledge software).

Protocols

Preparations were equilibrated in Tyrode’s solution for 60 to 90 minutes at 37°C until a stable baseline heart rate was achieved. The right vagus was placed through a pair of custom-built platinum ring electrodes and stimulated at 3, 5, 7, and 10 Hz (15 V, 1-ms pulse duration; order of stimulations randomized) for 25 seconds, with an interval of ≥1 minute between successive stimulations. In some experiments, vagal stimulation was repeated after application of the NO donor sodium nitroprusside ([SNP] 20 μmol/L, 10-minute incubation; Sigma). In addition, muscarinic responsiveness of atrial preparations was assessed using cumulative concentration–response curves to carbachol (0.1 to 0.5 μmol/L; Sigma; 2-minute incubation at each concentration).

Measurement of Acetylcholine Release

Experimental Preparation

Animals were killed and the right atria removed as described above. The preparation was then transferred to a preheated (37±0.2°C), continuously oxygenated, water-jacketed organ bath containing 4 mL of Tyrode’s solution where the atrium was pinned flat between 2 parallel silver stimulating electrodes 10 mm apart. Our methodology was similar to that described previously.16 After a 45-minute equilibration period (where the Tyrode’s solution was replaced every 15 minutes), the atrium was stimulated at 5 Hz (15 V, 1-ms pulse duration) for 1 minute and then again after another minute to stimulate acetylcholine (ACh) turnover. The preparation was then incubated for 30 minutes with [3H]choline chloride (10 μCi, Amersham United Kingdom) during which the atrium was stimulated at 5 Hz for 10 seconds every 30 seconds to incorporate the radiolabeled choline into parasympathetic transmitter stores. Tyrode’s solution containing 50 μmol/L of hemicholinium-3 (Sigma) was used after the incubation period to reduce reuptake of radioactively labeled transmitter. Excess [3H]choline was washed from the preparation by superfusing for 60 minutes at a rate of 3 mL/min with Tyrode’s solution.

Protocol

After the wash period, superfusion was stopped and the bath solution replaced every 3 minutes with a 0.5-mL sample being taken on every change of solution. This sample was added to 4.5 mL of scintillation fluid (Ecoscint A, National Diagnostics) and the amount of radioactivity in each sample (disintegrations per minute) measured using a liquid scintillation counter (Tri-carb 2800TR, Packard). After 16 and 94 minutes, the atrium was stimulated at 5 Hz for 1 minute, and after 34 and 112 minutes, it was stimulated again at 10 Hz for 1 minute (Figure 2A). In some experiments the sGC inhibitor 1H-1,2,4oxadiazolo(4,3-a)quinoxaline-1-one ([ODQ] 10 μmol/L; Sigma) was introduced to the solution after the first (control) 5-Hz stimulation and allowed to incubate for 45 minutes before the second 5-Hz stimulation was performed, whereas in additional experiments, SNP (20 μmol/L; Sigma) was added and allowed to incubate for 15 minutes before the second stimulation. At the end of the experiment, the atrium was immersed overnight in Tyrode’s solution containing 4 U/mL of papain (Sigma) and the radioactivity contained in the extract determined. [3H] outflow was expressed as a percentage of the total radioactivity in the atrium at the end of the experiment and that released after superfusion.

Measurement of Right Atrial cGMP Concentration

Experimental Preparation

Isolated, perfused, beating atria were prepared by methods described previously.19 In brief, the animal was killed and the right atrium removed as described above. A cannula containing 2 narrow bore catheters (4.5 mm OD) was inserted into the atrium and secured by ligatures. The outer tip of the atrial cannula was open to allow for outflow. The cannulated atrium was transferred to a preheated (36.5±0.2°C), continuously oxygenated, water-jacketed organ chamber and immediately perfused with oxygenated Tyrode’s solution by means of a peristaltic pump (0.5 mL/min).

Protocols

The atria were allowed to stabilize for 60 minutes. Once the stabilization period was completed, [3H]inulin (5 μCi, Amersham United Kingdom) was introduced to the pericardial fluid 20 minutes before the start of the sample collection to measure translocation of extracellular fluid. The perfusate was collected at 2-minute intervals at 4°C for analysis. Collections were performed during perfusion with Tyrode’s solution containing carbachol (0.3 μmol/L) for 10 minutes after a 20-minute control collection period and again after 10 minutes of washout with Tyrode’s solution.

Measurement of Extracellular Fluid Translocation

The radioactivity of [3H]inulin in atrial perfusate samples was measured with a liquid scintillation counter, and the amount of extracellular fluid translocated through the atrial wall was calculated, as described elsewhere.20

Radioimmunoassay of cGMP Concentration

For measurement of cGMP concentration in the atrial perfusate, 500 μL of the perfusate was treated with trichloroacetic acid to a final concentration of 6% for 15 minutes at room temperature and centrifuged at 4°C. The supernatant (200 μL) was extracted with water-saturated ether 3 times and then dried using a SpeedVac concentrator (Savant). The dried samples were resuspended, and a 125I-cGMP radioimmunoassay kit (Amersham United Kingdom) was used to measure the amount of cGMP after the bound form was separated from the free form by magnetic separation. The amount of cGMP efflux was expressed as picomoles of cGMP per minute per gram of atrial tissue. The molar concentration of cGMP in the interstitial space fluid21 was calculated as cGMP efflux concentration (nanomoles per liter)=cGMP (in picomoles per minute per gram)/extracellular fluid translocated (in microliters per minute per gram)×1000.

Right Atrial nNOS Gene Transfer and In Vivo Assessment of Cardiac Vagal Responsiveness

Gene Transfer Procedure

SHRs underwent gene transfer via percutaneous injection into the right atrium using methods similar to those described previously in the guinea pig.22 Animals were anesthetized with halothane (3% to 4% for induction and 2% to 3% for maintenance, in 100% O2) and injected with 5×1010 particles of replication-deficient adenoviral vector encoding nNOS (Ad.nNOS) or enhanced green fluorescent protein ([Ad.eGFP] control vector) in sterile PBS (300 μL injectate volume). The injection was performed using a 26-gauge needle, placed through the third intercostal space on the right side of the animal and directed toward the left axilla. Localization of the tip of the needle within the right atrial chamber was confirmed before injection by flashback of blood into the syringe, and the injection was performed during withdrawal of the needle from the atrial cavity. Phenotyping of transfected animals was performed ≈5 days after injection.

Anesthesia and Surgery

Surgical anesthesia was induced and maintained using halothane as described above, and a tracheostomy was performed to facilitate artificial ventilation (Harvard Rodent Ventilator, Model 683). After this, the left carotid artery and right jugular vein were cannulated (3FG and 2FG, respectively, Portex) for recording of blood pressure (SensoNor 840 pressure transducer) and infusion of fluids (4% dextran in 0.9% NaCl; Gentran, Baxter Healthcare Ltd) and drugs, respectively. In addition, subcutaneous stainless steel needle electrodes were placed for recording of the ECG. Heart rate was triggered from the blood pressure and ECG records and displayed in real time using a Biopac Systems MP100 data acquisition system (Biopac Systems Inc) and Acqknowledge software.

Intensive Care

Body temperature was monitored using a rectal thermocouple, and heating lamps placed above and below the animal were used to maintain body temperature within the range 37°C to 38°C. Arterial blood samples were regularly taken into preheparinized capillary tubes and used to measure blood gases and pH (ABL505, Radiometer Copenhagen); alteration of ventilatory parameters and/or infusion of 4.2% sodium bicarbonate solution (in 0.9% NaCl) was used to maintain blood gases and pH within acceptable limits (Pao2 >100 mm Hg; Paco2 35 to 45 mm Hg [pH 7.4±0.02]).

Experimental Protocol

Animals were bilaterally vagotomized, and the distal end of the right vagus was placed over a pair of hooked platinum stimulating electrodes. Vagal nerve stimulation was performed for 30 seconds at 3, 5, 7, and 10 Hz (15 V, 3-ms pulse duration; order of stimulations randomized), with an interval of ≥1 minute between successive stimulations. Rats were euthanized using an intravenous overdose of sodium pentobarbitone (Sagatal; Rhône Mérieux Ltd) on completion of the experimental protocol.

Measurement of sGC and nNOS Protein Expression and nNOS Activity

Western blotting for sGC and nNOS in right atria was performed using standard techniques as described previously,18 using commercially available polyclonal antibodies to α1-sGC (Sigma), nNOS (Zymed Laboratories Inc), and β-actin (Abcam plc) and the Western Lightening detection system (Perkin Elmer Life Sciences). Protein levels were expressed as a ratio of the optical densities of the nNOS/α1-sGC bands and the β-actin band to control for protein loading. Aorta and forebrain were used as positive controls for α1-sGC14 and nNOS,22 respectively. Protein (30 μg) was loaded into each lane.

Atrial NOS activity was quantified by measuring the conversion of [3H]-l-arginine to [3H]-l-citrulline using a modification of the procedure as described previously.23 Frozen atria were homogenized at 4°C in 200 μL of 50 mmol/L Tris (pH 7.5), containing 1 mmol/L of EDTA, 1 mmol/L of EGTA, 1 mmol/L of DTT, and protease inhibitor mixture (Sigma). After centrifuging the homogenate at 6000g for 5 minutes at 4°C, 50 μL of the supernatant was incubated in 200 μL of reaction buffer with a final concentration of 50 mmol/L of Tris (pH 7.5), 5 mmol/L of CaCl2, 1 mmol/L of MgCl2, 14 μmol/L of tetrahydrobiopterin, 10 μg/mL of calmodulin, 4 μmol/L of flavin adenine dinucleotide, 4 μmol/L of flavin adenine mononucleotide, 1 mmol/L of reduced nicotinamide adenine dinucleotide phosphate, and 1 μL of 1 mCi/mL [3H]-l-arginine. The activities of the nNOS isoforms were measured using a specific endothelial NO synthase inhibitor (l-N5-(1-Iminoethyl) ornithine, Dihydrochloride, Calbiochem Ltd) added to the assay buffer at a concentration of 10 μg per assay. After 30 minutes of incubation at 37°C, the reactions were stopped with 20 mmol/L of sodium acetate (pH 5.5), 0.2 mmol/L of EGTA, 1 mmol/L of l-citrulline, and 2 mmol/L of EDTA and poured over Dowex AG-50W-X8 columns (Bio-Rad). [3H]-l-citrulline was eluted with 2 mL of deionized water, and radioactivity was quantified by liquid scintillation counting. The results are expressed as femtomoles of citrulline per milligram of protein per minute.

Solutions and Drugs

Rat Tyrode’s solution contained (in mmol/L): NaCl 120, KCl 4.7, MgSO4 1.2, KH2PO4 1.2, NaHCO3 25, CaCl2 2, and glucose 11. The solution was constantly aerated (95% O2 and 5% CO2) to maintain pH at 7.4. All of the solutions were prepared fresh on the day of use using deionized water obtained from an Elga water purification system. Experiments using SNP were performed in a darkened room because of the light sensitivity of this drug.

Statistical Analysis

Data are presented as mean±SEM. Differences in the data were assessed using the t test or Mann–Whitney rank sum test as appropriate (SigmaStat, Systat Software Inc). Statistical significance was accepted at P<0.05.

Results

Rate Response to Vagal Nerve Stimulation

The heart rate response to right vagal stimulation was significantly reduced in the SHR (n=9) relative to the WKY (n=7) rats at 3, 5, and 7 Hz (P<0.05, unpaired t test; Figure 1A and 1B). No significant difference in responsiveness was observed at 10 Hz, although a trend toward impaired responsiveness in the SHR was present (P=0.07; see Figure 1).

Figure1
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Figure 1. A, Representative raw data traces showing impaired heart rate responsiveness to right vagal nerve stimulation in the SHR compared with the WKY rat. B, Graph showing significantly impaired responsiveness to 3-, 5-, and 7-Hz right vagal stimulation in the SHR (n=9) relative to the WKY rat (n=7; *P<0.05, unpaired t test).

Measurement of Right Atrial ACh Release

Release of [3H]ACh in response to right atrial field stimulation was significantly impaired at both 5 and 10 Hz in the SHR (n=6) compared with the WKY rats (n=7; 5 Hz: P<0.01; 10 Hz: P<0.05; unpaired t test; see Figure 2). S1 and S2 represent the first and second field stimulation, respectively. There was no difference between S1 and S2 at 5 or 10 Hz in either hypertensive or normotensive animals.

Figure2
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Figure 2. A and B, Typical data traces showing measurement of [3H]ACh release from isolated atria in response to 5- and 10-Hz field stimulation in WKY rats (A) and SHRs (B). S1 and S2 represent the first and second stimulation, respectively, at each frequency of stimulation. C, [3H]ACh release was significantly impaired at both 5- and 10-Hz field stimulation (S1) in the SHR (n=6) compared with the WKY (n=7; **P<0.01 and *P<0.05, respectively, unpaired t test).

Pharmacological Manipulation of NO-cGMP Pathway

Effect of NO Donor

Administration of 20 μmol/L of SNP significantly enhanced the release of [3H]ACh in response to 5-Hz field stimulation in the WKY rats (n=8; P<0.05, paired t test; see Figure 3A and 3C), whereas there was no effect in the SHRs (n=6; Figure 3B and 3C). This translated functionally where SNP significantly enhanced the rate responsiveness to vagal stimulation in the isolated double atrial preparation in the WKY rats (n=7; P<0.05, paired t test; Figure 3D). However, no response was seen in the SHRs (n=6) despite a similar increase in basal heart rate in the 2 strains (WKY: +49±7 [n=8] versus SHR: +43±6 [n=6] bpm) because of the well-established action of NO on the pacemaker itself.

Figure3
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Figure 3. A and B, Typical data trace showing effect of the NO donor, SNP (20 μmol/L), on [3H]ACh release during 5-Hz field stimulation in WKY rats (A) and SHRs (B). C, SNP significantly enhanced ACh release from the WKY rat (n=8; †P<0.05, paired t test) but not in the SHR (n=6). D, Heart rate responsiveness to vagal stimulation assessed using the double atrial preparation; SNP significantly increased vagal bradycardia in the WKY rat (n=7; †P<0.05, paired t test), whereas responsiveness was unaffected in the SHR (n=6; *P<0.05, **P<0.01, ***P<0.001, WKY vs SHR, unpaired t test).

Effect of sGC Inhibitor

Incubation with ODQ significantly decreased the release of [3H]ACh in response to 5-Hz field stimulation in the WKY (n=7; P<0.01, paired t test; see Figure 4). However, the release of [3H]ACh from SHR atria (n=7) was unaffected by ODQ.

Figure4
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Figure 4. A and B, Typical data traces showing the effect of sGC inhibition (ODQ; 10 μmol/L) on [3H]ACh release in response to 5-Hz field stimulation in WKY rats (A) and SHRs (B). C, ODQ significantly attenuated ACh release from the WKY rat (n=7; ††P<0.01, paired t test), whereas release from SHR atria was unaffected (n=7; **P<0.01, WKY vs SHR, unpaired t test).

Stimulation of Muscarinic Receptors

A comparison of cumulative concentration–response curves to carbachol revealed a significant increase in responsiveness in SHR atria at 0.1 and 0.5 μmol/L (P<0.05, unpaired t test; see Figure 5A for representative raw data traces and Figure 5B for grouped data). In perfused right atrial preparations, carbachol (0.3 μmol/L) also increased cGMP efflux concentration in the SHR (n=6), which was 21.5% higher than WKY responses (Figure 6; n=5; P<0.05, unpaired t test). In addition, atrial endothelial NO synthase activity was significantly greater in the SHR (SHR: 12.70±0.09 fmol/mg per minute versus WKY: 13.52±0.30 fmol/mg per minute), suggesting postsynaptic cellular remodeling of the receptor-coupled NO-cGMP pathway.

Figure5
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Figure 5. A, Raw data trace showing the heart rate response to cumulative additions of carbachol ([CCh] 0.1, 0.2, and 0.5 μmol/L) in double atrial preparations from WKY rats and SHRs. B, Responsiveness to 0.1 μmol/L (n=6) and 0.5 μmol/L (n=14). CCh was significantly increased in the SHR compared with WKY rat (0.1 μmol/L, n=7; 0.5 μmol/L, n=12; *P<0.01, unpaired t test). No significant difference was observed in response to 0.2 μmol/L of CCh (WKY, n=14; SHR, n=15).

Figure6
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Figure 6. Effect of CCh (0.3 μmol/L) on cGMP efflux concentration in perfused right atrial preparations from the SHR and WKY rats. No difference in basal cGMP efflux concentration was observed between WKY rats (n=5) and SHR (n=6). Administration of CCh significantly increased cGMP efflux concentration in the SHR (*P<0.05, unpaired t test) but not in the WKY.

nNOS Activity and Guanylate Cyclase Protein Levels After nNOS Gene Transfer

We evaluated the efficacy of Ad.nNOS gene transfer by measuring nNOS activity together with nNOS and α1-sGC protein expression in the atria of transfected WKY and SHR atria. Western blot analysis confirmed that there was a significant increase in both groups of rats in the intensity of the bands of nNOS (Figure 7A and 7B) and sGC (Figure 8) after Ad.nNOS compared with Ad.eGFP-treated rats. nNOS activity in Ad.nNOS-transfected rats was significantly increased in both groups compared with Ad.eGFP (WKY: +23.3±8.4%, P<0.05, unpaired t test; SHR: +15.7±3.2%, P<0.05, unpaired t test; Figure 7C).

Figure7
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Figure 7. Western blot analysis for nNOS in Ad.eGFP- and Ad.nNOS-transfected WKY (n=5 in each group) and SHR (n=7 in each group) atria. Protein sample (30 μg) was loaded in each lane. A, Visualized electrophoresis bands of nNOS and β-actin. B, Mean data of percentage changes of nNOS expression normalized to β-actin in Ad.eGFP- and Ad.nNOS-treated WKY rats (*P<0.05, unpaired t test) and SHRs (P<0.05, unpaired t test). C, nNOS activity measured in atria from Ad.GFP- and Ad.nNOS-transfected WKY (*P<0.05, unpaired t test; n=5 in each group) and SHR (*P<0.05, unpaired t test; n=7 in each group) atria.

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Figure 8. Western blot analysis for α1 subunit of guanylate cyclase (α1-sGC) in Ad.eGFP- and Ad.nNOS-transfected WKY (n=5 in each group) and SHR (n=7 in each group) atria. Protein sample (30 μg) was loaded in each lane. A, Visualized electrophoresis bands of α1-sGC and β-actin. B, Mean data of percentage changes of α1-sGC expression normalized to β-actin in Ad.eGFP- and Ad.nNOS-treated WKY rats (*P<0.05, unpaired t test) and SHRs (*P<0.05, unpaired t test).

Effects of nNOS Gene Transfer on Parasympathetic Function

A comparison of mean arterial pressures in Ad.eGFP- and Ad.nNOS-transfected SHRs showed no effect of nNOS gene transfer on blood pressure (126±3 mm Hg Ad.nNOS versus 116±6 mm Hg Ad.eGFP; n=6 for both groups). However, transfection with Ad.nNOS significantly increased vagal responsiveness in the SHR at all of the frequencies tested (eg, 3Hz: −40±3 bpm [Ad.nNOS, n=5] versus −19±4 [Ad.eGFP, n=7]; P=0.001, unpaired t test; Figure 9A).

Figure9
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Figure 9. A, Heart rate responses to 3- to 10-Hz right vagal stimulation in Ad.eGFP- (n=7 Embedded Image) and Ad.nNOS- (n=5 ▪) transfected SHRs. Vagal responsiveness was significantly enhanced by nNOS gene transfer at all of the frequencies tested (* P=0.001, unpaired t test; **P<0.001, Mann–Whitney rank sum test). B, Chronotropic responses of Ad.eGFP- (n=22 Embedded Image) and Ad.nNOS- (n=8 ▪) transfected SHR atria to carbachol (0.1 and 0.2 μmol/L). Responses to the muscarinic agonist were unaffected by nNOS gene transfer.

Rate responses of SHR atria to carbachol were unaffected by nNOS gene transfer at all of the concentrations tested (eg, 0.1 μmol/L: −29±2 bpm [Ad.eGFP, n=22] versus −28±4 [Ad.nNOS, n=8]; Figure 9B) suggesting that parasympathetic gain of function was occurring via facilitated presynpatic transmission. When in vivo SHR results were viewed quantitatively with the response of the WKY, it can be seen from Figure 10 that nNOS gene transfer enhanced vagal responsiveness in both groups of rats compared with the eGFP-treated controls. Moreover, nNOS-treated SHRs demonstrated a vagal response similar to that of eGFP-transfected WKYs at 3 and 5 Hz with an augmented response at 7 and 10 Hz.

Figure10
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Figure 10. Group data comparing heart rate responses to vagal nerve stimulation in vivo after Ad.eGFP and Ad.nNOS in the WKY and SHR. Gene transfer of nNOS significantly enhanced the rate response to vagal stimulation in the SHR compared with eGFP-treated SHR, augmenting the response to similar levels seen in Ad.eGFP-treated WKY treated at 3 and 5 Hz.

Discussion

The main findings of the current study are as follows: (1) at the level of the cardiac postganglionic neuron, bradycardia and acetylcholine release in response to vagal nerve stimulation are impaired in the SHRs compared with the normotensive WKY rats; (2) downregulation of the atrial NO-coupled guanylate cyclase pathway contributes to the parasympathetic phenotype in the SHR; and (3) upregulation of this pathway by gene transfer of adenoviral nNOS in the SHR restores peripheral cardiac vagal function in vivo.

Defective Peripheral Cardiac Vagal Function in the Hypertensive Rat

Increasing evidence supports the concept of sympathetic hyperactivity and parasympathetic underactivity as a significant component in the etiology of not only early and borderline hypertension but also in the maintenance of sustained essential hypertension.24–26 Furthermore, this is accompanied by a reduction in sensitivity of the parasympathetic component of the arterial baroreflex.27 The data presented here give direct evidence that vagally induced bradycardia and radiolabeled acetylcholine release are significantly impaired in the hypertensive rat at the level of the postganglionic cholinergic synapse. This demonstrates that a significant component of parasympathetic dysfunction occurs peripherally within efferent cardiac vagal neurons of the adult SHR. This is interesting in light of previous studies performed in young hypertensive rats in which others failed to observe an attenuation of cardiac vagal responsiveness.28

Our results also show that activation of postsynaptic muscarinic receptors in right atrial tissue with carbachol results in enhanced bradycardia and production of cGMP in the SHR, implicating hyperreactivity of the cholinergic system to a bath-applied agonist in this model. This may be related to the observation that atrial endothelial NO synthase activity is significantly higher in the SHR and, therefore, could lead to more NO-cGMP–dependent inhibition of pacemaking currents, like the inward calcium current ICaL.15 This could represent a postsynaptic compensatory mechanism resulting from the dysfunctional signaling at the prejunctional level.

Defective NO-cGMP Signaling in the Hypertensive Rat

Previous studies have shown that a presynaptic NO-cGMP pathway can modulate cardiac cholinergic transmission in adult guinea pig,16,29 rabbit,30 and nNOS knockout mice.31 Upregulation of nNOS expression within cardiac vagal neurons is essential for the increase in cardiac parasympathetic function observed as a result of aerobic exercise training.32,33 However, downregulation of sGC is observed in the aorta14 and right atrium15 of the SHR, where reduced atrial levels of cGMP are also observed.15

Attention has focused on the potential role of nNOS in the pathophysiology of hypertension based on its putative actions within the autonomic nervous system.34 It is well established that NO modulates activity within the central autonomic nuclei35 and that there may be defective nNOS-mediated signaling within the dorsal brain stem in hypertension.36 Our results show that an aspect of vagal impairment in the SHR resides within the peripheral nervous system at the level of the NO–sGC–cGMP pathway. Neither ODQ or SNP affected ACh release in the SHR, whereas ODQ could inhibit and SNP facilitate transmission in the WKY rat in a similar fashion to the normotensive guinea pig.16

Effect of nNOS Gene Transfer on Parasympathetic Function in the SHR

To determine whether our in vitro data were significant in the intact animals, heart rate responsiveness to vagal nerve stimulation was measured in anesthetized SHRs after adenoviral gene transfer of either nNOS or eGFP. nNOS-transfected animals displayed enhanced vagally mediated bradycardia at all of the frequencies of stimulation tested in comparison to eGFP-transfected animals. This result is consistent with those data published previously in the normotensive guinea pig and pig.17,18 We have reported recently that a similar gene transfer technique with nNOS increased nNOS protein expression and the production of right atrial tissue levels of cGMP in both the SHR and WKY rats.15 Consistent with this observation, we observed here that nNOS activity and the α1 subunit of sGC protein expression in WKY atria were significantly increased after Ad.nNOS, indicating that gene transfer upregulated NO bioavailability that then translated into vagal gain of function in both strains of rats.

The ability of nNOS gene overexpression to reverse peripheral vagal impairment in the hypertensive rat is somewhat surprising in light of our in vitro data from experiments using SNP. Administration of the NO donor SNP produced a prejunctional enhancement of cholinergic neurotransmission in right atrial preparations from the normotensive WKY rat. However, this response was absent in the atrial preparations from the SHR. It is possible that acute pharmacological administration of NO donors at a relatively low concentration (20 μmol/L) may not fully mimic the action of NO generated intracellularly by the nNOS transgene given its labile and highly reactive properties. In particular, a relatively high local concentration of NO may be required to increase the bioavailability of NO under conditions of increased oxidative stress that have been demonstrated previously in hypertension,12,13 because in the presence of the superoxide anion, NO will undergo rapid conversion to peroxynitrite. Indeed, evidence from experiments using synaptosomes isolated from the electric organ of Torpedo suggests that peroxynitrite may inhibit choline acetyltransferase and, therefore, reduce synthesis of ACh.37 It is, therefore, highly likely that nNOS gene transfer provided a more potent NO signal than the NO donor and that gene transfer also upregulated sCG in the SHR in a similar manner to our observations in WKY tissue.

Perspectives

The overall pathophysiological significance of these observations remains to be established. The vagus is nature’s cardiac calcium channel antagonist, and our data suggest that strategies designed to upregulate NO bioavailability or the downstream sites of sGC-cGMP (with NO synthase gene transfer) may be therapeutically beneficial in targeting the impaired parasympathetic phenotype associated with hypertension. Emerging evidence suggests that stimulating sGC inhibits cardiovascular remodeling in a model of hypertension.38 However, NO can have diverse effects within the nervous system where the spatial localization of the enzyme seems to be important for conferring specificity of action.39 This has been illustrated recently where NO has been shown to differentially modulate neurotransmission to premotor cardiac vagal neurons in the nucleus ambiguous.40 Therapeutically the brain is clearly a difficult structure to target with precision gene delivery. A realistic challenge would be to design a gene delivery system that specifically upregulates the NO synthase pathway in cardiac cholinergic ganglia and show that this strategy is effective in restoring neural control. Importantly, can it be demonstrated that this approach has an impact on the etiology of the disease itself.

In conclusion, we have established that a significant component of cardiac vagal impairment in the SHR resides at the end organ level because of abnormal NO-cGMP signaling in intracardiac ganglia. In a proof-of-principle study, nNOS gene transfer into these ganglia restores the neural phenotype by facilitating the release of ACh and improving cardiac parasympathetic function in vivo.

Acknowledgments

We are grateful to Dr Shijie Cai (Department of Cardiovascular Medicine, University of Oxford) for producing some of the adenoviral vector used in our experiments.

Sources of Funding

This work was supported by the British Heart Foundation (D.L. and D.A.H). S.C.A. was supported by a Corange Grant from Corpus Christi College, Oxford.

Disclosures

None.

Footnotes

  • The first 2 authors contributed equally to this work.

  • Received August 11, 2006.
  • Revision received September 4, 2006.
  • Accepted December 7, 2006.

References

  1. ↵
    Malliani A, Pagani M, Lombardi F, Furlan R, Guzzetti S, Cerutti S. Spectral analysis to assess increased sympathetic tone in arterial hypertension. Hypertension. 1991; 17: III36–III42.
    OpenUrlPubMed
  2. ↵
    Brook RD, Julius S. Autonomic imbalance, hypertension, and cardiovascular risk. Am J Hypertens. 2000; 13: 112S–122S.
    OpenUrlPubMed
  3. ↵
    Petretta M, Marciano F, Bianchi V, Migaux ML, Valva G, De Luca N, Salemme L, Berardino S, Bonaduce D. Power spectral analysis of heart period variability in hypertensive patients with left ventricular hypertrophy. Am J Hypertens. 1995; 8: 1206–1213.
    OpenUrlCrossRefPubMed
  4. ↵
    Langewitz W, Ruddel H, Schachinger H. Reduced parasympathetic cardiac control in patients with hypertension at rest and under mental stress. Am Heart J. 1994; 127: 122–128.
    OpenUrlCrossRefPubMed
  5. ↵
    Friberg P, Karlsson B, Nordlander M. Sympathetic and parasympathetic influence on blood pressure and heart rate variability in Wistar-Kyoto and spontaneously hypertensive rats. J Hypertens. 1988; 6 (suppl): S58–S60.
    OpenUrl
  6. ↵
    Murphy CA, Sloan RP, Myers MM. Pharmacologic responses and spectral analyses of spontaneous fluctuations in heart rate and blood pressure in shr rats. J Auton Nerv Syst. 1991; 36: 237–250.
    OpenUrlCrossRefPubMed
  7. ↵
    Piccirillo G, Viola E, Nocco M, Durante M, Tarantini S, Marigliano V. Autonomic modulation of heart rate and blood pressure in normotensive offspring of hypertensive subjects. J Lab Clin Med. 2000; 135: 145–152.
    OpenUrlCrossRefPubMed
  8. ↵
    Cole CR, Blackstone EH, Pashkow FJ, Snader CE, Lauer MS. Heart-rate recovery immediately after exercise as a predictor of mortality. N Engl J Med. 1999; 341: 1351–1357.
    OpenUrlCrossRefPubMed
  9. ↵
    La Rovere MT, Bigger JT Jr, Marcus FI, Mortara A, Schwartz PJ. Baroreflex sensitivity and heart-rate variability in prediction of total cardiac mortality after myocardial infarction. ATRAMI (autonomic tone and reflexes after myocardial infarction) investigators. Lancet. 1998; 351: 478–484.
    OpenUrlCrossRefPubMed
  10. ↵
    Vanoli E, De Ferrari GM, Stramba-Badiale M, Hull SS Jr, Foreman RD, Schwartz PJ. Vagal stimulation and prevention of sudden death in conscious dogs with a healed myocardial infarction. Circ Res. 1991; 68: 1471–1481.
    OpenUrlAbstract/FREE Full Text
  11. ↵
    Li M, Zheng C, Sato T, Kawada T, Sugimachi M, Sunagawa K. Vagal nerve stimulation markedly improves long-term survival after chronic heart failure in rats. Circulation. 2004; 109: 120–124.
    OpenUrlAbstract/FREE Full Text
  12. ↵
    Hamilton CA, Brosnan MJ, McIntyre M, Graham D, Dominiczak AF. Superoxide excess in hypertension and aging: a common cause of endothelial dysfunction. Hypertension. 2001; 37: 529–534.
    OpenUrlAbstract/FREE Full Text
  13. ↵
    Kerr S, Brosnan MJ, McIntyre M, Reid JL, Dominiczak AF, Hamilton CA. Superoxide anion production is increased in a model of genetic hypertension: Role of the endothelium. Hypertension. 1999; 33: 1353–1358.
    OpenUrlAbstract/FREE Full Text
  14. ↵
    Ruetten H, Zabel U, Linz W, Schmidt HH. Downregulation of soluble guanylyl cyclase in young and aging spontaneously hypertensive rats. Circ Res. 1999; 85: 534–541.
    OpenUrlAbstract/FREE Full Text
  15. ↵
    Heaton DA, Lei M, Li D, Golding S, Dawson TA, Mohan RM, Paterson DJ. Remodelling of cardiac pacemaker isoprenaline-stimulated L-type calcium current and β-adrenergic responsiveness in hypertension after neuronal no synthase gene transfer. Hypertension. 2006; 48: 443–452.
    OpenUrlAbstract/FREE Full Text
  16. ↵
    Herring N, Paterson DJ. Nitric oxide-cGMP pathway facilitates acetylcholine release and bradycardia during vagal nerve stimulation in the guinea-pig in vitro. J Physiol. 2001; 535: 507–518.
    OpenUrlCrossRefPubMed
  17. ↵
    Heaton DA, Golding S, Bradley CP, Dawson TA, Cai S, Channon KM, Paterson DJ. Targeted nnos gene transfer into the cardiac vagus rapidly increases parasympathetic function in the pig. J Mol Cell Cardiol. 2005; 39: 159–164.
    OpenUrlCrossRefPubMed
  18. ↵
    Mohan RM, Heaton DA, Danson EJ, Krishnan SP, Cai S, Channon KM, Paterson DJ. Neuronal nitric oxide synthase gene transfer promotes cardiac vagal gain of function. Circ Res. 2002; 91: 1089–1091.
    OpenUrlAbstract/FREE Full Text
  19. ↵
    Li D, Wen JF, Jin JY, Jin H, Ann HS, Kim SZ, Kim SH, Lee HS, Cho KW. Histamine inhibits atrial myocytic anp release via H2 receptor-camp-protein kinase signaling. Am J Physiol Regul Integr Comp Physiol. 2003; 285: R380–R393.
    OpenUrlAbstract/FREE Full Text
  20. ↵
    Cho KW, Kim SH, Hwang YH, Seul KH. Extracellular fluid translocation in perfused rabbit atria: Implication in control of atrial natriuretic peptide secretion. J Physiol. 1993; 468: 591–607.
    OpenUrlPubMed
  21. ↵
    Wen JF, Cui X, Jin JY, Kim SM, Kim SZ, Kim SH, Lee HS, Cho KW. High and low gain switches for regulation of camp efflux concentration: Distinct roles for particulate GC- and soluble GC-cGMP-PDE3 signaling in rabbit atria. Circ Res. 2004; 94: 936–943.
    OpenUrlAbstract/FREE Full Text
  22. ↵
    Mohan RM, Choate JK, Golding S, Herring N, Casadei B, Paterson DJ. Peripheral pre-synaptic pathway reduces the heart rate response to sympathetic activation following exercise training: Role of NO. Cardiovasc Res. 2000; 47: 90–98.
    OpenUrlAbstract/FREE Full Text
  23. ↵
    Wehling-Henricks M, Jordan MC, Roos KP, Deng B, Tidball JG. Cardiomyopathy in dystrophin-deficient hearts is prevented by expression of a neuronal nitric oxide synthase transgene in the myocardium. Hum Mol Genet. 2005; 14: 1921–1933.
    OpenUrlAbstract/FREE Full Text
  24. ↵
    Julius S, Pascual AV, London R. Role of parasympathetic inhibition in the hyper-kinetic type of borderline hypertension. Circulation. 1971; 44: 413–418.
    OpenUrlAbstract/FREE Full Text
  25. ↵
    Cabral AM, Vasquez EC. Time course of cardiac sympathetic and vagal tone changes in renovascular hypertensive rats. Am J Hypertens. 1991; 4: 815–819.
    OpenUrlPubMed
  26. ↵
    Dabrowska B, Dabrowski A, Skrobowski A. Parasympathetic withdrawal precedes spontaneous blood pressure elevations in women with primary hypertension. Cardiology. 1996; 87: 119–124.
    OpenUrlCrossRefPubMed
  27. ↵
    Pitzalis MV, Passantino A, Massari F, Forleo C, Balducci C, Santoro G, Mastropasqua F, Antonelli G, Rizzon P. Diastolic dysfunction and baroreflex sensitivity in hypertension. Hypertension. 1999; 33: 1141–1145.
    OpenUrlAbstract/FREE Full Text
  28. ↵
    Ferrari AU, Daffonchio A, Franzelli C, Mancia G. Cardiac parasympathetic hyperresponsiveness in spontaneously hypertensive rats. Hypertension. 1992; 19: 653–657.
    OpenUrlPubMed
  29. ↵
    Herring N, Golding S, Paterson DJ. Pre-synaptic no-cgmp pathway modulates vagal control of heart rate in isolated adult guinea pig atria. J Mol Cell Cardiol. 2000; 32: 1795–1804.
    OpenUrlCrossRefPubMed
  30. ↵
    Sears CE, Choate JK, Paterson DJ. No-cgmp pathway accentuates the decrease in heart rate caused by cardiac vagal nerve stimulation. J Appl Physiol. 1999; 86: 510–516.
    OpenUrlAbstract/FREE Full Text
  31. ↵
    Choate JK, Danson EJ, Morris JF, Paterson DJ. Peripheral vagal control of heart rate is impaired in neuronal nos knockout mice. Am J Physiol Heart Circ Physiol. 2001; 281: H2310–H2317.
    OpenUrlAbstract/FREE Full Text
  32. ↵
    Danson EJ, Mankia KS, Golding S, Dawson T, Everatt L, Cai S, Channon KM, Paterson DJ. Impaired regulation of neuronal nitric oxide synthase and heart rate during exercise in mice lacking one nnos allele. J Physiol. 2004; 558: 963–974.
    OpenUrlCrossRefPubMed
  33. ↵
    Danson EJ, Paterson DJ. Enhanced neuronal nitric oxide synthase expression is central to cardiac vagal phenotype in exercise-trained mice. J Physiol. 2003; 546: 225–232.
    OpenUrlCrossRefPubMed
  34. ↵
    Chowdhary S, Townend JN. Nitric oxide and hypertension: not just an endothelium derived relaxing factor! J Hum Hypertension. 2001; 15: 219–227.
    OpenUrlCrossRefPubMed
  35. ↵
    Ma S, Abboud FM, Felder RB. Effects of L-arginine-derived nitric oxide synthesis on neuronal activity in nucleus tractus solitarius. Am J Physiol. 1995; 268: R487–R491.
    OpenUrlPubMed
  36. ↵
    Pontieri V, Venezuela MK, Scavone C, Michelini LC. Role of endogenous nitric oxide in the nucleus tratus solitarii on baroreflex control of heart rate in spontaneously hypertensive rats. J Hypertens. 1998; 16: 1993–1999.
    OpenUrlCrossRefPubMed
  37. ↵
    Morot Gaudry-Talarmain Y, Moulian N, Meunier FA, Blanchard B, Angaut-Petit D, Faille L, Ducrocq C. Nitric oxide and peroxynitrite affect differently acetylcholine release, choline acetyltransferase activity, synthesis, and compartmentation of newly formed acetylcholine in torpedo marmorata synaptosomes. Nitric Oxide. 1997; 1: 330–345.
    OpenUrlCrossRefPubMed
  38. ↵
    Masuyama H, Tsuruda T, Kato J, Imamura T, Asada Y, Stasch J-S, Kitamura K, Eto T. Soluble guanylate cyclase stimulation on cardiovascular remodelling in angiotensin II-induced hypertensive rats. Hypertension. 2006; 48: 972–978.
    OpenUrlAbstract/FREE Full Text
  39. ↵
    Paton JF, Kasparov S, Paterson DJ. Nitric oxide and autonomic control of heart rate: A question of specificity. Trends Neurosci. 2002; 25: 626–631.
    OpenUrlCrossRefPubMed
  40. ↵
    Kamendi H, Dergacheva O, Wang X, Huang ZG, Bouairi E, Gorini C, Mendelowitz D. NO differentially regulates neurotransmission to premotor cardiac vagal neurons in the nucleus ambiguus. Hypertension. 2006; 48: 1137–1142.
    OpenUrlAbstract/FREE Full Text
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February 2007, Volume 49, Issue 2
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    Gene Transfer of Neuronal Nitric Oxide Synthase into Intracardiac Ganglia Reverses Vagal Impairment in Hypertensive Rats
    Daniel A. Heaton, Dan Li, Susanna C. Almond, Tom A. Dawson, Lijun Wang, Keith M. Channon and David J. Paterson
    Hypertension. 2007;49:380-388, originally published January 18, 2007
    https://doi.org/10.1161/01.HYP.0000255792.97033.f7

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    Gene Transfer of Neuronal Nitric Oxide Synthase into Intracardiac Ganglia Reverses Vagal Impairment in Hypertensive Rats
    Daniel A. Heaton, Dan Li, Susanna C. Almond, Tom A. Dawson, Lijun Wang, Keith M. Channon and David J. Paterson
    Hypertension. 2007;49:380-388, originally published January 18, 2007
    https://doi.org/10.1161/01.HYP.0000255792.97033.f7
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