Intrarenal Angiotensin III Infusion Induces Natriuresis and Angiotensin Type 2 Receptor Translocation in Wistar-Kyoto but not in Spontaneously Hypertensive Rats
This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.
In Sprague-Dawley rats, renal angiotensin (Ang) type 2 receptors (AT2Rs) mediate natriuresis in response to renal interstitial (RI) D1-like receptor stimulation or RI Ang III infusion. After D1-like receptor activation, apical membrane (AM) but not total renal proximal tubule cell AT2R expression is increased, suggesting that AM AT2R translocation may be important for natriuresis. The onset of hypertension in spontaneously hypertensive rats (SHRs) is preceded by defects in renal sodium excretion. The present study examines AT2R-mediated natriuresis in response to RI Ang III infusion in Wistar-Kyoto rats (WKYs) and SHRs. WKYs and SHRs received RI Ang III infusion after 24 hours of systemic AT1R blockade with candesartan. In WKYs, urine sodium excretion rate increased from 0.043±0.01 to 0.191±0.06 μmol/min (P<0.05) in response to Ang III infusion, but identical conditions failed to increase the urine sodium excretion rate in SHRs. The increase in the urine sodium excretion rate was blocked by coinfusion of PD-123319, a selective AT2R antagonist. On confocal microscopy images, Ang III–infused WKYs demonstrated greater renal proximal tubule cell AM AT2R fluorescence intensity compared with SHRs (5385±725 versus 919±35; P<0.0001), and Western blot analysis demonstrated increased AM (0.050±0.003 versus 0.038±0.003; P<0.01) but not total cell AT2R expression in WKYs. In SHRs, AM AT2R expression remained unchanged in response to RI Ang III infusion. Thus, RI Ang III infusion elicits natriuresis and renal proximal tubule cell AT2R translocation in WKYs. Identical manipulations fail to induce natriuresis or AT2R translocation in SHRs, suggesting that defects in AT2R-mediated natriuresis and trafficking may be important to the development of hypertension in SHRs.
- Received October 3, 2008.
- Revision received October 31, 2008.
- Accepted November 20, 2008.