In the Hypertension article by Marques et al (Marques FZ, Campain AE, Yang YHJ, Morris BJ. Meta-Analysis of Genome-Wide Gene Expression Differences in Onset and Maintenance Phases of Genetic Hypertension. Hypertension. 2010;56:319–324), a transposition error was made by one of us (A.E.C.) in the entry of values for 26 of the 58 sets of microarray data for hypertensive and normotensive rats in the maintenance phase of hypertension. As a result, the gene lists generated for the maintenance phase were incorrect. A corrected online supplement with changes highlighted has been uploaded. Those genes having the biggest differences in expression in the false discovery rate (FDR) analysis remain. In contrast, many with smaller expression differences are no longer present. The correlation between the new and old results in the FDR analysis is 0.65.
After Bonferroni correction (P<0.05), we now find 27 genes with significant differential expression (Table S4). In the FDR analysis (P<0.05), there were 169 with significant differential expression (Table S5). Of these 169 genes, 71 were present in the original FDR analysis. And of these 71 genes, 8 were in common with the 27 in the new Bonferroni analysis. The correct analysis now implicates the genes C5ar1, Ccl2, Eno1, Ephx2, Mx1, Pf4, Pla2g2a, Postn, Reg3b, Thbs4, and Uts2r, amongst others, in the maintenance phase of hypertension. This new gene list showed an over-representation of gene ontology terms for inflammatory response and chemokine activity, down-regulation of angiogenesis, regulation of body fluid levels, G-coupled protein receptor binding, and calcium ion binding (Table S6).
All data for the onset phase of hypertension remain correct as published.
The authors apologize for the error in the original maintenance phase data analysis.
These corrections have been made to the current online data supplement of the article, which is available at http://hyper.ahajournals.org/cgi/content/full/56/9/319.
- © 2011 American Heart Association, Inc.