Abstract 187: A Postnatal Diet Rich in Fat and Sucrose Leads to the Differential Alterations in Renal Function and Metabolic Health in Male Control and Intrauterine Growth Restricted Offspring
We previously reported that reduced uterine perfusion induced at day 14 of gestation in the rat programs a marked increase in mean arterial pressure (MAP) in male intrauterine growth restricted (IUGR) offspring compared to male control with no impact on renal function or metabolic health. Recent studies suggest that the degree of mismatch between the pre- and postnatal environments can alter the programming of adult health and disease. This study tested the hypothesis that IUGR followed by exposure to a diet rich in fat and sucrose would exacerbate programmed increases in blood pressure, alter renal function and impair metabolic health in male IUGR offspring. Control and IUGR offspring were maintained on regular rodent chow (RC) or switched to high fat/high sucrose (HF/HS) diet at delivery and maintained on the RC or HF/HS diet for 6 months (control RC: n=12, IUGR RC: n=9, control HF/HS: n=13, IUGR HF/HS n=12). MAP, measured in conscious, chronically instrumented via arterial catheter, was significantly increased by postnatal exposure to a HF/HS diet in both control and IUGR offspring (149.7±3 versus 150.6±3 mmHg, control HF/HS versus IUGR HF/HS, respectively); thus, normalizing the significant difference in MAP observed in control versus IUGR offspring on RC. Glomerular filtration rate (GFR) adjusted per kidney weight did not differ in control RC or IUGR RC. However, it was significantly reduced in IUGR HF/HS relative to control HF/HS (1.9±0.5 vs. 0.3±0.1 mg/min/gram kidney; P<0.05, control HF/HS versus IUGR HF/HS, respectively) indicating that despite no difference in MAP in the HF/HS groups, a mismatch of pre- and post-natal diet altered renal function in IUGR offspring. Represented by area under the curve (AUC), an impaired oral glucose tolerance test was observed in control HF/HS relative to all other groups (P<0.05); AUC did not differ in IUGR HF/HS relative to offspring on RC (control RC: 436.6±15 versus IUGR RC: 464.0±27, control HF/HS: 552.2±23, and IUGR HF/HS 469.7±23). Thus, male IUGR offspring were protected from impaired metabolic health following postnatal exposure to HF/HS diet. These findings indicate that male control and IUGR offspring exhibit a differential response to postnatal exposure to a HF/HS diet on renal function and metabolic health.
- © 2012 by American Heart Association, Inc.