Abstract 245: Synergistic Effects of Nicotine and Angiotensin II: Implications for Progression of Chronic Kidney Disease in Smokers
Increased generation of Angiotensin II (ANG II) plays a key role in the progression of chronic kidney disease (CKD). Cigarette smoking accelerates the progression of CKD of different etiologies including hypertension and diabetes. We have shown that nicotine (NIC), a biologically active compound present in large amounts in tobacco, worsens the severity of injury in experimental models of CKD. The aim of this study is to determine whether NIC and ANG II have synergistic effects on fibronectin production and proliferation in mesangial cells (MC). Rat MC were exposed to different concentrations of NIC (10-4 M to 10-10 M) and/or ANG II (10-6 M to 10-10 M) for 24 hours. Fibronectin production was measured by western blot and proliferation by EdU incorporation. As shown in Table 1, NIC and ANG II have synergistic effects on the production of fibronectin in MC.
Shown in Table 2, NIC and ANG II have additive effects on MC proliferation.
These synergistic effects of NIC and ANG II may explain the deleterious effects of smoking in conditions associated with increased activation of the renin angiotensin system.
- © 2012 by American Heart Association, Inc.