Abstract 568: Congenic Mapping of Hypertension in the Nephrectomy Model of Chronic Kidney Disease.
Introduction: We previously reported a locus on mouse chromosome 11 (chrom 11) linked to development of hypertension (HTN) after sub-total nephrectomy (Nx). To begin fine mapping, we used the 129S6 (129) susceptible strain and the C57BL/6 (B6) resistant strain to generate 4 reciprocal congenic lines carrying ~ 2-LOD intervals of the linked locus for recombinant progeny testing. The intervals are defined by microsatellite markers 1-5 and markers 1-6, corresponding to regions defined by markers D11Mit2 and D11Mit177, and D11Mit2 and D11Mit285, respectively. Through brother-sister matings, the 4 lines are as follows: 1) 129 background, homozygous for B6 segment defined by markers 1-5 (129Chrom11:1-5ofB6), 2) 129 background, homozygous for 129 segment defined by markers 1-5 (129Chrom11:1-5of129), 3) B6 background, homozygous for 129 segment defined by markers 1-6 (B6Chrom11:1-6of129) and 4) B6 background, homozygous for B6 segment defined by markers 1-6 (B6Chrom11:1-6ofB6).
Methods: Sub-total Nx was performed on male and female congenic mice at ~ 8 weeks of age. Beginning 4 weeks after surgery, using tail cuff manometer, mice were trained for 2 weeks, after which systolic blood pressure (SBP) was recorded daily for 2 weeks.
Results: Table 1 summarizes the average SBP of the congenic lines.
Conclusion: These findings confirm the effect of this chrom 11 region is dependent on the 129 genetic background, and suggest that interactions between this 129 locus and other loci in the 129 genome are necessary for the development of HTN after nephron mass reduction. Moreover, the influence of this region on chrom 11 appears to be dependent on sex.
Author Disclosures: S. Cechova: None. R. Chan: None. C. Rudy: None. T.H. Le: B. Research Grant (includes principal investigator, collaborator, or consultant and pending grants as well as grants already received); Modest; NIDDK grant # DK0795035-01A2.
- © 2014 by American Heart Association, Inc.