Abstract MP10: Nobelty and Significance of the Rapid Measurement of Plasma Aldosterone Concentration and Active Renin Concentration
The plasma aldosterone/renin ratio (ARR) for the clinical diagnosis of primary aldosteronism (PA) is calculated with plasma aldosterone concentration (PAC) per plasma renin activity (PRA) or active renin concentration (ARC). It takes 3-4 days to get the results. Of late, we developed the novel rapid non-RIA assays of PAC and ARC, which are measurable in 10 minutes. Both were simultaneously measured by chemiluminescent enzyme immunoassay (CLEIA) system machine with their specific monoclonal antibodies and were automatically washed by the immobilized magnetic particles. We retrospectively compared RIA-assayed PAC,PRA and ARC with CLEIA-measured PAC and ARC in 243 patients with aldosterone producing adenoma (APA, n=86), bilateral hyperaldosteronism (BHA, n=78) and essential hypertension (EH, n=79), and investigated whether the calculation of ARR with ARC (ARR-C, ng/dL per pg/mL) instead of PRA (ARR-A, ng/dL per ng/m/hour) affects the diagnosing of the APA patients, which are potentially surgically curable. CLEIA-measured PAC were significantly correlated with RIA-assayed PAC (y=0.9846 x + 2.5708, Spearman's r =0.9072, P<0.0001). CLEIA-measured ARC (the lower detection limit = 0.25 pg/mL) were also significantly correlated with RIA-assayed ARC (y=1.0103 x + 0.9156, Spearman's r =0.8166, P<0.0001). ARR-A and ARR-C of APA patients were 129 ± 4.3 and 25.1±2.7 (Mean ± SEM), respectively. ARR-A and ARR-C of BHA patients were 40.4 ± 2.9 and 6.7 ± 0.5, and those of EH patients 17.2 ± 2.1 and 3.2 ± 0.4, respectively. Our novel rapid CLEIA-assays of PAC and ARC will be able to be clinically more useful for detecting APA or BHA because it takes only ten minutes to get the results.
Author Disclosures: F. Satoh: None. Y. Ono: None. R. Morimoto: None. Y. Iwakura: None. M. Nezu: None. K. Omata: None. Y. Tezuka: None. M. Kudo: None. K. Seiji: None. K. Takase: None. Y. Nakamura: None. C.E. Gomez-Sanchez: None. H. Sasano: None. S. Ito: None.
- © 2015 by American Heart Association, Inc.