Abstract P136: What is the Impact of Arterial Stiffness on Brain's Health
Arterial stiffness is an important risk factor for cognitive decline. However, its specific effects on brain homeostasis are unknown. Hence, the objective of the study is to explore the effects of arterial stiffness on brain's health, especially on oxidative stress, inflammation, cerebrovascular regulation and cognitive functions.
Approach and Results: Arterial stiffness was induced by applying calcium chloride to carotid arteries of C57BL6 male mice. The control group received sodium chloride. Cerebral inflammation was assessed by quantifying immunoreactivity to activated glia markers ; Iba-1, CD68 and s100β. Oxidative stress was determined with dihydroethidium. Cerebral blood flow (CBF) was monitored by laser-Doppler flowmetry in anesthetized mice equipped with a cranial window and spatial memory was tested using the Morris water maze. Results show that arterial stiffness activates microglia in the hippocampus, and astrocytes in the hippocampus and the frontal cortex. Superoxide anion production was elevated in the hippocampus of these mice. Arterial stiffness attenuated the CBF increase produced by stimulation of the vibrissae or by topical application of the endothelium-dependent vasodilator acetylcholine. The results indicate learning and spatial memory deficit induced by arterial stiffness in comparison to the sodium chloride group.
Conclusions: This study shows that arterial stiffness, induced by carotid calcification, leads to cerebral inflammation and increased oxidative stress mainly in the hippocampus. Arterial stiffness also alters CBF regulation and cognitive functions. This suggests that arterial stiffness has an impact on cerebral homeostasis and should be considered as a therapeutical target for the prevention of cerebral dysfunctions in the aging population.
Author Disclosures: H. Girouard: None. D. Obari: None. G. Muhire: None. D. Vallerand: None. G. Ferland: None. N. Sadekova: None.
- © 2015 by American Heart Association, Inc.