Abstract P643: Proliferative Potential of Perivascular Adipose Tissue Stromal Vascular Cells Is Dependent on Anatomical Site
Perivascular adipose tissue (PVAT) is an important paracrine regulator of blood vessel function. Growth and pathological conditions such as obesity expand PVAT by hyperplasia and hypertrophy, however, these remodeling processes may differ depending on the PVAT anatomical site leading to diverse effects on the vasculature. A higher proliferative capacity of PVAT localized around mesenteric arteries may contribute to increased visceral fat mass and therefore intensify CVD risk. We hypothesize that PVAT proliferative potential is dependent on PVAT anatomical localization. PVATs from aorta (aPVAT) and mesenteric arteries (mPVAT) were collected from male Sprague Dawley rats at 10 weeks of age (n=5). Visceral depots including gonadal (GON) and retroperitoneal (RP), and the subcutaneous inguinal pad (SC) were collected as non-perivascular adipose controls. Stromal vascular fraction cells (SVF) from each adipose site were harvested and flow cytometry was performed to assess their expression of surface markers for committed preadipocyte precursors including CD34, CD44, and CD140a. Cells co-expressing these markers have high adipogenic capacity and are highly proliferative in visceral adipose tissues during obesity. No differences among sites were observed in the percentage of SVF cells expressing CD34 (aPVAT 39.3%±9.1; mPVAT 46.4%±11.16; GON 51.15%±14.11; RP 37.68%±5.6; SC 29.74%±2.5) and CD140a (aPVAT 1.43%±0.65; mPVAT 3.54%±1.33; GON 3.53%±0.36; RP 1.95%±0.91; SC 2.42%±0.81). There was a higher number of CD44+ SVF in mPVAT (3.93%±0.8) and GON (4.4%±1.11) compared to aPVAT, RP, and SC (1.4%±0.13; 1.53%±0.53; 2.08±0.37. P<0.05). Proliferation capacity of SVF was evaluated by plating 2x105 cells/cm2 of PVATs and GON, as highly proliferative and adipogenic control site, supplemented with DMEM:F12 media (10%FBS). At 11 days in culture, the number of SVF/cm2 was significantly lower in aPVAT (7.04x105 cells/cm2) vs. mPVAT and GON (13.75 x105 and 12.62 x105 cells/cm2; P<0.05). These data demonstrate a site dependent proliferation capacity of SVF cells from PVATs that may be explained in part by differences in the cellular distribution of adipocyte progenitors.
Author Disclosures: G. Contreras: None. K. Thelen: None. N. Ayala-Lopez: None. S.W. Watts: None.
- © 2015 by American Heart Association, Inc.