Skip to main content
  • American Heart Association
  • Science Volunteer
  • Warning Signs
  • Advanced Search
  • Donate

  • Home
  • About this Journal
    • General Statistics
    • Editorial Board
    • Editors
    • Information for Advertisers
    • Author Reprints
    • Commercial Reprints
    • Customer Service and Ordering Information
  • All Issues
  • Subjects
    • All Subjects
    • Arrhythmia and Electrophysiology
    • Basic, Translational, and Clinical Research
    • Critical Care and Resuscitation
    • Epidemiology, Lifestyle, and Prevention
    • Genetics
    • Heart Failure and Cardiac Disease
    • Hypertension
    • Imaging and Diagnostic Testing
    • Intervention, Surgery, Transplantation
    • Quality and Outcomes
    • Stroke
    • Vascular Disease
  • Browse Features
    • AHA Guidelines and Statements
    • Acknowledgment of Reviewers
    • Clinical Implications
    • Clinical-Pathological Conferences
    • Controversies in Hypertension
    • Editors' Picks
    • Guidelines Debate
    • Meeting Abstracts
    • Recent Advances in Hypertension
    • SPRINT Trial: the Conversation Continues
  • Resources
    • Instructions to Reviewers
    • Instructions for Authors
    • →Article Types
    • → Submission Guidelines
      • Research Guidelines
        • Minimum Information About Microarray Data Experiments (MIAME)
      • Abstract
      • Acknowledgments
      • Clinical Implications (Only by invitation)
      • Conflict(s) of Interest/Disclosure(s) Statement
      • Figure Legends
      • Figures
      • Novelty and Significance: 1) What Is New, 2) What Is Relevant?
      • References
      • Sources of Funding
      • Tables
      • Text
      • Title Page
      • Online/Data Supplement
    • →Tips for Easier Manuscript Submission
    • → General Instructions for Revised Manuscripts
      • Change of Authorship Form
    • → Costs to Authors
    • → Open Access, Repositories, & Author Rights Q&A
    • Permissions to Reprint Figures and Tables
    • Journal Policies
    • Scientific Councils
    • AHA Journals RSS Feeds
    • International Users
    • AHA Newsroom
  • AHA Journals
    • AHA Journals Home
    • Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB)
    • Circulation
    • → Circ: Arrhythmia and Electrophysiology
    • → Circ: Genomic and Precision Medicine
    • → Circ: Cardiovascular Imaging
    • → Circ: Cardiovascular Interventions
    • → Circ: Cardiovascular Quality & Outcomes
    • → Circ: Heart Failure
    • Circulation Research
    • Hypertension
    • Stroke
    • Journal of the American Heart Association
  • Facebook
  • Twitter

  • My alerts
  • Sign In
  • Join

  • Advanced search

Header Publisher Menu

  • American Heart Association
  • Science Volunteer
  • Warning Signs
  • Advanced Search
  • Donate

Hypertension

  • My alerts
  • Sign In
  • Join

  • Facebook
  • Twitter
  • Home
  • About this Journal
    • General Statistics
    • Editorial Board
    • Editors
    • Information for Advertisers
    • Author Reprints
    • Commercial Reprints
    • Customer Service and Ordering Information
  • All Issues
  • Subjects
    • All Subjects
    • Arrhythmia and Electrophysiology
    • Basic, Translational, and Clinical Research
    • Critical Care and Resuscitation
    • Epidemiology, Lifestyle, and Prevention
    • Genetics
    • Heart Failure and Cardiac Disease
    • Hypertension
    • Imaging and Diagnostic Testing
    • Intervention, Surgery, Transplantation
    • Quality and Outcomes
    • Stroke
    • Vascular Disease
  • Browse Features
    • AHA Guidelines and Statements
    • Acknowledgment of Reviewers
    • Clinical Implications
    • Clinical-Pathological Conferences
    • Controversies in Hypertension
    • Editors' Picks
    • Guidelines Debate
    • Meeting Abstracts
    • Recent Advances in Hypertension
    • SPRINT Trial: the Conversation Continues
  • Resources
    • Instructions to Reviewers
    • Instructions for Authors
    • →Article Types
    • → Submission Guidelines
    • →Tips for Easier Manuscript Submission
    • → General Instructions for Revised Manuscripts
    • → Costs to Authors
    • → Open Access, Repositories, & Author Rights Q&A
    • Permissions to Reprint Figures and Tables
    • Journal Policies
    • Scientific Councils
    • AHA Journals RSS Feeds
    • International Users
    • AHA Newsroom
  • AHA Journals
    • AHA Journals Home
    • Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB)
    • Circulation
    • → Circ: Arrhythmia and Electrophysiology
    • → Circ: Genomic and Precision Medicine
    • → Circ: Cardiovascular Imaging
    • → Circ: Cardiovascular Interventions
    • → Circ: Cardiovascular Quality & Outcomes
    • → Circ: Heart Failure
    • Circulation Research
    • Hypertension
    • Stroke
    • Journal of the American Heart Association
Original Article

MicroRNA-376c Impairs Tumor Growth Factor-β and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion

Guodong Fu, Gang Ye, Lubna Nadeem, Lei Ji, Tanita Manchanda, Yongqing Wang, Yangyu Zhao, Jie Qiao, Yan-Ling Wang, Stephen Lye, Burton B. Yang, Chun Peng
Download PDF
https://doi.org/10.1161/HYPERTENSIONAHA.111.203489
Hypertension. 2013;HYPERTENSIONAHA.111.203489
Originally published February 19, 2013
Guodong Fu
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gang Ye
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lubna Nadeem
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lei Ji
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tanita Manchanda
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yongqing Wang
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yangyu Zhao
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jie Qiao
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yan-Ling Wang
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen Lye
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Burton B. Yang
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chun Peng
From the Department of Biology, York University, Toronto, Ontario, Canada (G.F., G.Y., L.N., T.M., C.P.); State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (L.J., Y-L.W.); Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China (Y.W., Y.Z., J.Q.); Research Center for Women’s and Infants Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada (S.L.); and Sunnybrook Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada (B.B.Y.).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Supplemental Materials
  • Info & Metrics
  • eLetters

Jump to

  • Article
  • Supplemental Materials
  • Info & Metrics
  • eLetters
Loading

Abstract

Preeclampsia is a major disorder of pregnancy and a leading cause of maternal and perinatal morbidity and mortality. MicroRNAs are small noncoding RNAs that regulate gene expression posttranscriptionally. In this study, we examined the expression of miR-376c and found that miR-376c levels were downregulated in both placental and plasma samples collected from preeclamptic patients, when compared with the normal pregnant women at the same gestational stage. Overexpression of miR-376c–induced trophoblast cell proliferation, migration, and invasion in HTR8/SVneo cells and promoted placental explant outgrowth. In contrast, inhibition of endogenous miR-376c resulted in a decrease in trophoblast cell invasion and placental explant outgrowth. We identified activin receptor-like kinase 5 (ALK5), a type I receptor for transforming growth factor-β, and ALK7, a type I receptor for Nodal, as targets of miR-376c. Overexpression of miR-376c repressed transforming growth factor-β and Nodal functions, whereas overexpression of ALK5 and ALK7 reversed the effects of miR-376c. These results demonstrate that miR-376c inhibits both ALK5 and ALK7 expression to impair transforming growth factor-β/Nodal signaling, leading to increases in cell proliferation and invasion. An unbalanced Nodal/transforming growth factor-β and miR-376c expression may lead to the development of preeclampsia.

  • ALK5
  • ALK7
  • miR-376c
  • Nodal
  • placenta
  • preeclampsia
  • TGF-β
  • Received August 3, 2012.
  • Revision received December 19, 2012.
  • Accepted January 28, 2013.
  • © 2013 American Heart Association, Inc.
Back to top
Next Article

Current Issue

Hypertension
May 2018, Volume 71, Issue 5
  • Table of Contents
Next Article

Jump to

  • Article
  • Supplemental Materials
  • Info & Metrics
  • eLetters

Article Tools

  • Print
  • Citation Tools
    MicroRNA-376c Impairs Tumor Growth Factor-β and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion
    Guodong Fu, Gang Ye, Lubna Nadeem, Lei Ji, Tanita Manchanda, Yongqing Wang, Yangyu Zhao, Jie Qiao, Yan-Ling Wang, Stephen Lye, Burton B. Yang and Chun Peng
    Hypertension. 2013;HYPERTENSIONAHA.111.203489, originally published February 19, 2013
    https://doi.org/10.1161/HYPERTENSIONAHA.111.203489

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
  • Article Alerts
    Log in to Email Alerts with your email address.
  • Save to my folders

Share this Article

  • Email

    Thank you for your interest in spreading the word on Hypertension.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    MicroRNA-376c Impairs Tumor Growth Factor-β and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion
    (Your Name) has sent you a message from Hypertension
    (Your Name) thought you would like to see the Hypertension web site.
  • Share on Social Media
    MicroRNA-376c Impairs Tumor Growth Factor-β and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion
    Guodong Fu, Gang Ye, Lubna Nadeem, Lei Ji, Tanita Manchanda, Yongqing Wang, Yangyu Zhao, Jie Qiao, Yan-Ling Wang, Stephen Lye, Burton B. Yang and Chun Peng
    Hypertension. 2013;HYPERTENSIONAHA.111.203489, originally published February 19, 2013
    https://doi.org/10.1161/HYPERTENSIONAHA.111.203489
    del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo

Related Articles

Cited By...

Subjects

  • Cardiology
    • Etiology
      • Hypertension
        • Hypertension

Hypertension

  • About Hypertension
  • Instructions for Authors
  • AHA CME
  • Guidelines and Statements
  • Permissions
  • Journal Policies
  • Email Alerts
  • Open Access Information
  • AHA Journals RSS
  • AHA Newsroom

Editorial Office Address:
7272 Greenville Ave.
Dallas, TX 75231
email: hypertension@heart.org

Information for:
  • Advertisers
  • Subscribers
  • Subscriber Help
  • Institutions / Librarians
  • Institutional Subscriptions FAQ
  • International Users
American Heart Association Learn and Live
National Center
7272 Greenville Ave.
Dallas, TX 75231

Customer Service

  • 1-800-AHA-USA-1
  • 1-800-242-8721
  • Local Info
  • Contact Us

About Us

Our mission is to build healthier lives, free of cardiovascular diseases and stroke. That single purpose drives all we do. The need for our work is beyond question. Find Out More about the American Heart Association

  • Careers
  • SHOP
  • Latest Heart and Stroke News
  • AHA/ASA Media Newsroom

Our Sites

  • American Heart Association
  • American Stroke Association
  • For Professionals
  • More Sites

Take Action

  • Advocate
  • Donate
  • Planned Giving
  • Volunteer

Online Communities

  • AFib Support
  • Garden Community
  • Patient Support Network
  • Professional Online Network

Follow Us:

  • Follow Circulation on Twitter
  • Visit Circulation on Facebook
  • Follow Circulation on Google Plus
  • Follow Circulation on Instagram
  • Follow Circulation on Pinterest
  • Follow Circulation on YouTube
  • Rss Feeds
  • Privacy Policy
  • Copyright
  • Ethics Policy
  • Conflict of Interest Policy
  • Linking Policy
  • Diversity
  • Careers

©2018 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited. The American Heart Association is a qualified 501(c)(3) tax-exempt organization.
*Red Dress™ DHHS, Go Red™ AHA; National Wear Red Day ® is a registered trademark.

  • PUTTING PATIENTS FIRST National Health Council Standards of Excellence Certification Program
  • BBB Accredited Charity
  • Comodo Secured