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Original Article

Angiotensin II Stimulates Basolateral 10-pS Cl Channels in the Thick Ascending Limb

Peng Wu, Mingxiao Wang, Haiyan Luan, Lili Li, Lijun Wang, Wen-Hui Wang, Ruimin Gu
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https://doi.org/10.1161/HYPERTENSIONAHA.111.01069
Hypertension. 2013;HYPERTENSIONAHA.111.01069
Originally published April 8, 2013
Peng Wu
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Mingxiao Wang
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Haiyan Luan
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Lili Li
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Lijun Wang
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Wen-Hui Wang
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Ruimin Gu
From the Department of Pharmacology (P.W., M.W., H.L., L.L., R.G.) and Department of Physiology (L.W.), Harbin Medical University, Harbin, China; and Department of Pharmacology, New York Medical College, Valhalla, NY (L.W., W.H.W.).
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Abstract

Chloride channels in the basolateral membrane play a key role in Cl absorption in the thick ascending limb (TAL). The patch-clamp experiments were performed to test whether angiotensin II (AngII) increases Cl absorption in the TAL by stimulating the basolateral 10-pS Cl channels. AngII (1–100 nmol/L) stimulated the 10-pS Cl channel in the TAL, an effect that was blocked by losartan (angiotension AT1 receptor [AT1R] antagonist) but not by PD123319 (angiotension AT2 receptor [AT2R] antagonist). Inhibition of phospholipase C or protein kinase C also abolished the stimulatory effect of AngII on Cl channels. Moreover, stimulation of protein kinase C with phorbol-12-myristate-13-acetate mimicked the effect of AngII and increased Cl channel activity. However, the stimulatory effect of AngII on Cl channels was absent in the TAL pretreated with diphenyleneiodonium sulfate, an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Moreover, treatment of the TAL with diphenyleneiodonium sulfate also blocked the effect of phorbol-12-myristate-13-acetate on the 10-pS Cl channel. Western blotting demonstrated that incubation of isolated TAL with AngII increased phosphorylation of p47phox at Ser304, suggesting that AngII stimulates the basolateral Cl channels by increasing NADPH oxidase–dependent superoxide generation. This notion was also supported by the observation that H2O2 significantly increased 10-pS Cl channel activity in the TAL. We conclude that stimulation of AT1R increased the basolateral Cl channels by activating the protein kinase C–dependent NADPH oxidase pathway. The stimulatory effect of AngII on the basolateral Cl channel may contribute to AngII-induced increases in NaCl reabsorption in the TAL and AngII-infuse–induced hypertension.

  • angiotensin II receptor
  • ClC-Kb channel
  • hypertension
  • NADPH oxidase
  • protein kinase C
  • Received January 16, 2013.
  • Revision received March 8, 2013.
  • Accepted March 9, 2013.
  • © 2013 American Heart Association, Inc.
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    Angiotensin II Stimulates Basolateral 10-pS Cl Channels in the Thick Ascending Limb
    Peng Wu, Mingxiao Wang, Haiyan Luan, Lili Li, Lijun Wang, Wen-Hui Wang and Ruimin Gu
    Hypertension. 2013;HYPERTENSIONAHA.111.01069, originally published April 8, 2013
    https://doi.org/10.1161/HYPERTENSIONAHA.111.01069

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    Angiotensin II Stimulates Basolateral 10-pS Cl Channels in the Thick Ascending Limb
    Peng Wu, Mingxiao Wang, Haiyan Luan, Lili Li, Lijun Wang, Wen-Hui Wang and Ruimin Gu
    Hypertension. 2013;HYPERTENSIONAHA.111.01069, originally published April 8, 2013
    https://doi.org/10.1161/HYPERTENSIONAHA.111.01069
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