Table 3.

Effect of Sex and Juvenile Obesity on Gene Expression Markers of Glucocorticoid Action, Inflammation, Lipid Sensing, Cell Adhesion and Renal Function Apoptosis, Cell Proliferation, and Glucose Metabolism in the Kidneys of Young Adult Sheep

Pathway
GeneLean Female (n=7)Obese Female (n=9)Lean Male (n=11)Obese Male (n=9)Effect of Sex
Glucocorticoid action11βHSD20.88±0.190.81±0.110.76±0.121.85±0.25*O
GR0.86±0.161.29±0.232.27±0.2515.46±1.56†O
ProinflammationCCR20.78±0.091.11±0.173.98±0.597.42±0.82†LO
IFNγ1.35±0.371.27±0.2615.98±1.8721.43±7.48O
IL-62.07±0.371.98±0.231.07±0.141.13±0.14L
IL-181.60±0.441.12±0.201.02±0.041.20±0.24NS
MCP-12.53±0.541.98±0.231.81±0.172.77±0.31NS
TNFα0.12±0.030.07±0.010.12±0.020.14±0.01O
Anti-inflammationIL-100.85±0.250.86±0.022.71±0.063.07±0.04O
iNOS0.85±0.020.09±0.020.16±0.030.23±0.03O
PPARγ0.16±0.040.16±0.200.29±0.020.37±0.08O
Lipid sensing receptorsCD143.98±1.125.21±1.0911.06±2.4526.96±7.81†O
TLR44.41±0.645.34±0.9117.24±2.2733.11±5.89*O
Cell adhesion moleculesICAM-11.69±0.601.23±0.152.56±0.344.20±0.72O
VCAM-10.10±0.040.11±0.020.20±0.020.35±0.05*O
Renal functionEPOR0.31±0.090.43±0.080.54±0.101.09±0.13*O
Renin0.39±0.190.26±0.030.29±0.050.40±0.10NS
  • 11βHSD2 indicates 11β-hydroxysteroid dehydrogenase type 2; GR, glucocorticoid receptor (type 2); CCR2, C-C motif receptor 2; IFN, interferon; IL, interleukin; MCP, monocyte chemoattractant protein; TNF, tumor necrosis factor; iNOS, inducible nitric oxide synthase; PPARγ, peroxisome proliferator activated receptor γ; CD14, cluster of differentiation 14; TLR4, toll-like receptor 4; ICAM, intercellular adhesion molecule; VCAM, vascular cell adhesion molecule; EPOR, erythropoietin receptor; L, effect of sex between lean groups where P<0.005; O effect of sex between obese groups where P<0.005; NS, no significant difference.

  • Values are mean±SEM.

  • Effect of obesity: *P<0.005 and †P<0.001.